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KMID : 0352719980220020126
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Journal of Ginseng Research
1998 Volume.22 No. 2 p.126 ~ p.132
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Effects of Ginsenosides on pp60c-src Kinase, Intracellular Calcium and Cell Proliferation in NIH 373 Cells
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Hee-Youn Hong
Gyurng-Soo Yoo/Jung-Kap Choi
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Abstract
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In the present study, we examined effects of ginseng saponins (ginsenosides) on pp60c-src protein tyrosine kinase (PTK) activity, intracellular calcium concentration ([Ca2+]i), and cell proliferation in NIH3T3 cells. Eight different ginsenosides [ginsenoside-Rb1 (G-Rb1), -Rb2, -Rc, -Rd, -Re, -Rf, -Rg1, -Rg2) and ginseng total saponin (GTS) were used for these experiments. All ginsenosides and GTS tested stimulated the activation of pp60c?src kinase, and especially G-Rb1,-Rd,-Rg1, and -Rg1 showed a higher stimulatory effect than others at 16.7 μg/ml of ginsenosides with a 18 hr-incubation, increasing the activity by 4.5, 3.5, 3.5, and 3.0-fold, respectively, over that of untreated control. In addition, both G-Rd and -Rg2)Rg2 increased (Ca2+), to 202 and 334 nM, respectively, about 2-3-fold above the basal level within 7min at 250 μg/yml of ginsenosides. The increases of (Ca2+), were eliminated by Pretreatment of EGTA, an extracellular calcium chelator, suggtasting that they result from an influx of calcium ion from extracellular medium rather than an efflux from intracellular calcium store, endoplasmic reticulum (ER). All ginsenosides studied enhanced cell proliferation to 1.2-1.4-fold over that of untreated control at 5~250 μg/ml of concentrations. Interestingly the promotion of cell proliferation by ginsenosides corresponded with the activation of c-src kinase, which is an early step in the mitogenic signaling cascade. Taken together, we suggest that some ginsenosides may lead to cellProliferation via the activation of cellular signal transduction Pathway involving pp60c?src kinase.
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